adipogenic induction medium Search Results


90
Lonza adipogenic media
Adipogenic Media, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Lonza adipogenic induction and maintance medium
Adipogenic Induction And Maintance Medium, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Procell Inc adipogenic differentiation basic medium a
Adipogenic Differentiation Basic Medium A, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Lonza preadipocyte basal medium bulletkit
(A) Schematic workflow for constructing vascularized adipose spheroids from the inguinal white adipose tissue (iWAT) in mice. Isolated stromal vascular fraction (SVF) is seeded in an ultra-low attachment (ULA) plate in endothelial growth medium (blue dots). The spheroids are embedded in extracellular matrix (ECM) at day (D)6, adipogenesis is induced at D10 by exchanging the medium to <t>preadipocyte</t> growth medium (grey dots) and the spheroids are ready to use at day 21. (B) Light microscopy images for morphological assessment of spheroids embedded +/- ECM from D9, 15 and 21. Immunofluorescence staining for the endothelial marker CD31 (red) in spheroids +/- ECM at D21, which have been merged with counterstained nuclei (blue) using Hoechst, and Bodipy to identify lipids (green). Scalebars 200 and 400 μM. (C) Quantification of spheroid surface area at the indicated days. Each dot represents one spheroid, n = 3 per time point, per condition. (D) Relative mRNA expression of adipocyte markers, Fabp4 and Adipoq , and endothelium markers, Cdh5 and Rbp7 in spheroids – ECM (grey) and + ECM (blue). n = 3 per condition. The mRNA expressions were normalized to Gtf2b expression and presented as means ± standard deviation (SD). Statistics were calculated using unpaired, non-parametric t -test followed by Mann-Whitney, # indicates p = 0.1.
Preadipocyte Basal Medium Bulletkit, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/preadipocyte basal medium bulletkit/product/Lonza
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preadipocyte basal medium bulletkit - by Bioz Stars, 2026-03
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Cambrex adipogenic induction and maintenance medium
(A) Schematic workflow for constructing vascularized adipose spheroids from the inguinal white adipose tissue (iWAT) in mice. Isolated stromal vascular fraction (SVF) is seeded in an ultra-low attachment (ULA) plate in endothelial growth medium (blue dots). The spheroids are embedded in extracellular matrix (ECM) at day (D)6, adipogenesis is induced at D10 by exchanging the medium to <t>preadipocyte</t> growth medium (grey dots) and the spheroids are ready to use at day 21. (B) Light microscopy images for morphological assessment of spheroids embedded +/- ECM from D9, 15 and 21. Immunofluorescence staining for the endothelial marker CD31 (red) in spheroids +/- ECM at D21, which have been merged with counterstained nuclei (blue) using Hoechst, and Bodipy to identify lipids (green). Scalebars 200 and 400 μM. (C) Quantification of spheroid surface area at the indicated days. Each dot represents one spheroid, n = 3 per time point, per condition. (D) Relative mRNA expression of adipocyte markers, Fabp4 and Adipoq , and endothelium markers, Cdh5 and Rbp7 in spheroids – ECM (grey) and + ECM (blue). n = 3 per condition. The mRNA expressions were normalized to Gtf2b expression and presented as means ± standard deviation (SD). Statistics were calculated using unpaired, non-parametric t -test followed by Mann-Whitney, # indicates p = 0.1.
Adipogenic Induction And Maintenance Medium, supplied by Cambrex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Biowit Technologies chondrogenic differentiation medium
(A) Schematic workflow for constructing vascularized adipose spheroids from the inguinal white adipose tissue (iWAT) in mice. Isolated stromal vascular fraction (SVF) is seeded in an ultra-low attachment (ULA) plate in endothelial growth medium (blue dots). The spheroids are embedded in extracellular matrix (ECM) at day (D)6, adipogenesis is induced at D10 by exchanging the medium to <t>preadipocyte</t> growth medium (grey dots) and the spheroids are ready to use at day 21. (B) Light microscopy images for morphological assessment of spheroids embedded +/- ECM from D9, 15 and 21. Immunofluorescence staining for the endothelial marker CD31 (red) in spheroids +/- ECM at D21, which have been merged with counterstained nuclei (blue) using Hoechst, and Bodipy to identify lipids (green). Scalebars 200 and 400 μM. (C) Quantification of spheroid surface area at the indicated days. Each dot represents one spheroid, n = 3 per time point, per condition. (D) Relative mRNA expression of adipocyte markers, Fabp4 and Adipoq , and endothelium markers, Cdh5 and Rbp7 in spheroids – ECM (grey) and + ECM (blue). n = 3 per condition. The mRNA expressions were normalized to Gtf2b expression and presented as means ± standard deviation (SD). Statistics were calculated using unpaired, non-parametric t -test followed by Mann-Whitney, # indicates p = 0.1.
Chondrogenic Differentiation Medium, supplied by Biowit Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Beijing Solarbio Science adipogenic induction liquid
(A) Schematic workflow for constructing vascularized adipose spheroids from the inguinal white adipose tissue (iWAT) in mice. Isolated stromal vascular fraction (SVF) is seeded in an ultra-low attachment (ULA) plate in endothelial growth medium (blue dots). The spheroids are embedded in extracellular matrix (ECM) at day (D)6, adipogenesis is induced at D10 by exchanging the medium to <t>preadipocyte</t> growth medium (grey dots) and the spheroids are ready to use at day 21. (B) Light microscopy images for morphological assessment of spheroids embedded +/- ECM from D9, 15 and 21. Immunofluorescence staining for the endothelial marker CD31 (red) in spheroids +/- ECM at D21, which have been merged with counterstained nuclei (blue) using Hoechst, and Bodipy to identify lipids (green). Scalebars 200 and 400 μM. (C) Quantification of spheroid surface area at the indicated days. Each dot represents one spheroid, n = 3 per time point, per condition. (D) Relative mRNA expression of adipocyte markers, Fabp4 and Adipoq , and endothelium markers, Cdh5 and Rbp7 in spheroids – ECM (grey) and + ECM (blue). n = 3 per condition. The mRNA expressions were normalized to Gtf2b expression and presented as means ± standard deviation (SD). Statistics were calculated using unpaired, non-parametric t -test followed by Mann-Whitney, # indicates p = 0.1.
Adipogenic Induction Liquid, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
adipogenic induction liquid - by Bioz Stars, 2026-03
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Lonza osteogenic and adipogenic induction/maintenance medium
(A) Schematic workflow for constructing vascularized adipose spheroids from the inguinal white adipose tissue (iWAT) in mice. Isolated stromal vascular fraction (SVF) is seeded in an ultra-low attachment (ULA) plate in endothelial growth medium (blue dots). The spheroids are embedded in extracellular matrix (ECM) at day (D)6, adipogenesis is induced at D10 by exchanging the medium to <t>preadipocyte</t> growth medium (grey dots) and the spheroids are ready to use at day 21. (B) Light microscopy images for morphological assessment of spheroids embedded +/- ECM from D9, 15 and 21. Immunofluorescence staining for the endothelial marker CD31 (red) in spheroids +/- ECM at D21, which have been merged with counterstained nuclei (blue) using Hoechst, and Bodipy to identify lipids (green). Scalebars 200 and 400 μM. (C) Quantification of spheroid surface area at the indicated days. Each dot represents one spheroid, n = 3 per time point, per condition. (D) Relative mRNA expression of adipocyte markers, Fabp4 and Adipoq , and endothelium markers, Cdh5 and Rbp7 in spheroids – ECM (grey) and + ECM (blue). n = 3 per condition. The mRNA expressions were normalized to Gtf2b expression and presented as means ± standard deviation (SD). Statistics were calculated using unpaired, non-parametric t -test followed by Mann-Whitney, # indicates p = 0.1.
Osteogenic And Adipogenic Induction/Maintenance Medium, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Bioscientifica Ltd adipogenic induction medium
(A) Schematic workflow for constructing vascularized adipose spheroids from the inguinal white adipose tissue (iWAT) in mice. Isolated stromal vascular fraction (SVF) is seeded in an ultra-low attachment (ULA) plate in endothelial growth medium (blue dots). The spheroids are embedded in extracellular matrix (ECM) at day (D)6, adipogenesis is induced at D10 by exchanging the medium to <t>preadipocyte</t> growth medium (grey dots) and the spheroids are ready to use at day 21. (B) Light microscopy images for morphological assessment of spheroids embedded +/- ECM from D9, 15 and 21. Immunofluorescence staining for the endothelial marker CD31 (red) in spheroids +/- ECM at D21, which have been merged with counterstained nuclei (blue) using Hoechst, and Bodipy to identify lipids (green). Scalebars 200 and 400 μM. (C) Quantification of spheroid surface area at the indicated days. Each dot represents one spheroid, n = 3 per time point, per condition. (D) Relative mRNA expression of adipocyte markers, Fabp4 and Adipoq , and endothelium markers, Cdh5 and Rbp7 in spheroids – ECM (grey) and + ECM (blue). n = 3 per condition. The mRNA expressions were normalized to Gtf2b expression and presented as means ± standard deviation (SD). Statistics were calculated using unpaired, non-parametric t -test followed by Mann-Whitney, # indicates p = 0.1.
Adipogenic Induction Medium, supplied by Bioscientifica Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/adipogenic induction medium/product/Bioscientifica Ltd
Average 90 stars, based on 1 article reviews
adipogenic induction medium - by Bioz Stars, 2026-03
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ScienCell adipogenic-conditioned media
(A) Schematic workflow for constructing vascularized adipose spheroids from the inguinal white adipose tissue (iWAT) in mice. Isolated stromal vascular fraction (SVF) is seeded in an ultra-low attachment (ULA) plate in endothelial growth medium (blue dots). The spheroids are embedded in extracellular matrix (ECM) at day (D)6, adipogenesis is induced at D10 by exchanging the medium to <t>preadipocyte</t> growth medium (grey dots) and the spheroids are ready to use at day 21. (B) Light microscopy images for morphological assessment of spheroids embedded +/- ECM from D9, 15 and 21. Immunofluorescence staining for the endothelial marker CD31 (red) in spheroids +/- ECM at D21, which have been merged with counterstained nuclei (blue) using Hoechst, and Bodipy to identify lipids (green). Scalebars 200 and 400 μM. (C) Quantification of spheroid surface area at the indicated days. Each dot represents one spheroid, n = 3 per time point, per condition. (D) Relative mRNA expression of adipocyte markers, Fabp4 and Adipoq , and endothelium markers, Cdh5 and Rbp7 in spheroids – ECM (grey) and + ECM (blue). n = 3 per condition. The mRNA expressions were normalized to Gtf2b expression and presented as means ± standard deviation (SD). Statistics were calculated using unpaired, non-parametric t -test followed by Mann-Whitney, # indicates p = 0.1.
Adipogenic Conditioned Media, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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adipogenic-conditioned media - by Bioz Stars, 2026-03
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Lonza adipogenic-inducting medium
(A) Schematic workflow for constructing vascularized adipose spheroids from the inguinal white adipose tissue (iWAT) in mice. Isolated stromal vascular fraction (SVF) is seeded in an ultra-low attachment (ULA) plate in endothelial growth medium (blue dots). The spheroids are embedded in extracellular matrix (ECM) at day (D)6, adipogenesis is induced at D10 by exchanging the medium to <t>preadipocyte</t> growth medium (grey dots) and the spheroids are ready to use at day 21. (B) Light microscopy images for morphological assessment of spheroids embedded +/- ECM from D9, 15 and 21. Immunofluorescence staining for the endothelial marker CD31 (red) in spheroids +/- ECM at D21, which have been merged with counterstained nuclei (blue) using Hoechst, and Bodipy to identify lipids (green). Scalebars 200 and 400 μM. (C) Quantification of spheroid surface area at the indicated days. Each dot represents one spheroid, n = 3 per time point, per condition. (D) Relative mRNA expression of adipocyte markers, Fabp4 and Adipoq , and endothelium markers, Cdh5 and Rbp7 in spheroids – ECM (grey) and + ECM (blue). n = 3 per condition. The mRNA expressions were normalized to Gtf2b expression and presented as means ± standard deviation (SD). Statistics were calculated using unpaired, non-parametric t -test followed by Mann-Whitney, # indicates p = 0.1.
Adipogenic Inducting Medium, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/adipogenic-inducting medium/product/Lonza
Average 90 stars, based on 1 article reviews
adipogenic-inducting medium - by Bioz Stars, 2026-03
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STEMCELL Technologies Inc adipogenic induction medium #05412
(A) Schematic workflow for constructing vascularized adipose spheroids from the inguinal white adipose tissue (iWAT) in mice. Isolated stromal vascular fraction (SVF) is seeded in an ultra-low attachment (ULA) plate in endothelial growth medium (blue dots). The spheroids are embedded in extracellular matrix (ECM) at day (D)6, adipogenesis is induced at D10 by exchanging the medium to <t>preadipocyte</t> growth medium (grey dots) and the spheroids are ready to use at day 21. (B) Light microscopy images for morphological assessment of spheroids embedded +/- ECM from D9, 15 and 21. Immunofluorescence staining for the endothelial marker CD31 (red) in spheroids +/- ECM at D21, which have been merged with counterstained nuclei (blue) using Hoechst, and Bodipy to identify lipids (green). Scalebars 200 and 400 μM. (C) Quantification of spheroid surface area at the indicated days. Each dot represents one spheroid, n = 3 per time point, per condition. (D) Relative mRNA expression of adipocyte markers, Fabp4 and Adipoq , and endothelium markers, Cdh5 and Rbp7 in spheroids – ECM (grey) and + ECM (blue). n = 3 per condition. The mRNA expressions were normalized to Gtf2b expression and presented as means ± standard deviation (SD). Statistics were calculated using unpaired, non-parametric t -test followed by Mann-Whitney, # indicates p = 0.1.
Adipogenic Induction Medium #05412, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/adipogenic induction medium #05412/product/STEMCELL Technologies Inc
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Image Search Results


(A) Schematic workflow for constructing vascularized adipose spheroids from the inguinal white adipose tissue (iWAT) in mice. Isolated stromal vascular fraction (SVF) is seeded in an ultra-low attachment (ULA) plate in endothelial growth medium (blue dots). The spheroids are embedded in extracellular matrix (ECM) at day (D)6, adipogenesis is induced at D10 by exchanging the medium to preadipocyte growth medium (grey dots) and the spheroids are ready to use at day 21. (B) Light microscopy images for morphological assessment of spheroids embedded +/- ECM from D9, 15 and 21. Immunofluorescence staining for the endothelial marker CD31 (red) in spheroids +/- ECM at D21, which have been merged with counterstained nuclei (blue) using Hoechst, and Bodipy to identify lipids (green). Scalebars 200 and 400 μM. (C) Quantification of spheroid surface area at the indicated days. Each dot represents one spheroid, n = 3 per time point, per condition. (D) Relative mRNA expression of adipocyte markers, Fabp4 and Adipoq , and endothelium markers, Cdh5 and Rbp7 in spheroids – ECM (grey) and + ECM (blue). n = 3 per condition. The mRNA expressions were normalized to Gtf2b expression and presented as means ± standard deviation (SD). Statistics were calculated using unpaired, non-parametric t -test followed by Mann-Whitney, # indicates p = 0.1.

Journal: Frontiers in Endocrinology

Article Title: Mouse vascularized adipose spheroids: an organotypic model for thermogenic adipocytes

doi: 10.3389/fendo.2024.1396965

Figure Lengend Snippet: (A) Schematic workflow for constructing vascularized adipose spheroids from the inguinal white adipose tissue (iWAT) in mice. Isolated stromal vascular fraction (SVF) is seeded in an ultra-low attachment (ULA) plate in endothelial growth medium (blue dots). The spheroids are embedded in extracellular matrix (ECM) at day (D)6, adipogenesis is induced at D10 by exchanging the medium to preadipocyte growth medium (grey dots) and the spheroids are ready to use at day 21. (B) Light microscopy images for morphological assessment of spheroids embedded +/- ECM from D9, 15 and 21. Immunofluorescence staining for the endothelial marker CD31 (red) in spheroids +/- ECM at D21, which have been merged with counterstained nuclei (blue) using Hoechst, and Bodipy to identify lipids (green). Scalebars 200 and 400 μM. (C) Quantification of spheroid surface area at the indicated days. Each dot represents one spheroid, n = 3 per time point, per condition. (D) Relative mRNA expression of adipocyte markers, Fabp4 and Adipoq , and endothelium markers, Cdh5 and Rbp7 in spheroids – ECM (grey) and + ECM (blue). n = 3 per condition. The mRNA expressions were normalized to Gtf2b expression and presented as means ± standard deviation (SD). Statistics were calculated using unpaired, non-parametric t -test followed by Mann-Whitney, # indicates p = 0.1.

Article Snippet: The isolated inguinal white adipose tissue stromal vascular fraction was resuspended in Preadipocyte Basal Medium (BulletKit, Lonza, PT-8002) with the addition of L-glutamine, GA-1000 and FBS (SingleQuots, part of BulletKit, Lonza, PT-8002) and seeded in 12–24-wells (Thermo Scientific, 150628 and 142475) depending on the experiment.

Techniques: Isolation, Light Microscopy, Immunofluorescence, Staining, Marker, Expressing, Standard Deviation, MANN-WHITNEY