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Image Search Results
Journal: Frontiers in Endocrinology
Article Title: Mouse vascularized adipose spheroids: an organotypic model for thermogenic adipocytes
doi: 10.3389/fendo.2024.1396965
Figure Lengend Snippet: (A) Schematic workflow for constructing vascularized adipose spheroids from the inguinal white adipose tissue (iWAT) in mice. Isolated stromal vascular fraction (SVF) is seeded in an ultra-low attachment (ULA) plate in endothelial growth medium (blue dots). The spheroids are embedded in extracellular matrix (ECM) at day (D)6, adipogenesis is induced at D10 by exchanging the medium to preadipocyte growth medium (grey dots) and the spheroids are ready to use at day 21. (B) Light microscopy images for morphological assessment of spheroids embedded +/- ECM from D9, 15 and 21. Immunofluorescence staining for the endothelial marker CD31 (red) in spheroids +/- ECM at D21, which have been merged with counterstained nuclei (blue) using Hoechst, and Bodipy to identify lipids (green). Scalebars 200 and 400 μM. (C) Quantification of spheroid surface area at the indicated days. Each dot represents one spheroid, n = 3 per time point, per condition. (D) Relative mRNA expression of adipocyte markers, Fabp4 and Adipoq , and endothelium markers, Cdh5 and Rbp7 in spheroids – ECM (grey) and + ECM (blue). n = 3 per condition. The mRNA expressions were normalized to Gtf2b expression and presented as means ± standard deviation (SD). Statistics were calculated using unpaired, non-parametric t -test followed by Mann-Whitney, # indicates p = 0.1.
Article Snippet: The isolated inguinal white adipose tissue stromal vascular fraction was resuspended in
Techniques: Isolation, Light Microscopy, Immunofluorescence, Staining, Marker, Expressing, Standard Deviation, MANN-WHITNEY